FastQCFastQC Report
Sun 14 Apr 2019
SRR6575974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156714
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1830.1167732302155519No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA1830.1167732302155519No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1770.11294459971668135No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC1750.11166838955039116No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT1740.11103028446724607No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1630.10401112855265005No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA1610.10273491838635987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA950.059.455421
GTATCAA2850.057.803881
TCAACGC4000.039.9447144
ATCAACG4300.038.250753
TTTACAC651.4090238E-436.149063
CAACGCA4450.035.9053575
AACGCAG4450.035.9053576
TTATGCA550.002711646534.177294
TATCAAC5000.032.895642
CATGGGG4300.032.7863584
CGCAGAG5100.032.2712258
GTACATG6600.032.092411
TACATGG6700.031.5629862
ACGCAGA5250.030.4340677
CATAGTA804.765972E-429.371112
ACATGGG7200.029.371113
TAAACTG650.006142152528.9192495
GCAGAGT5650.028.2974999
AGAGTAC4950.027.54929210-11
GTATAAT909.4001635E-426.1493741
Produced by FastQC (version 0.11.2)