FastQCFastQC Report
Sun 14 Apr 2019
SRR6575971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245146
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16030.1287399228684829No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15300.12287715657440974No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15050.12086935989835731No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14310.11492628173724206No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13940.11195474265668444No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13170.10577072889444292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18650.061.682861
GGTATCA6750.047.4961741
ATCAACG27500.041.1917463
TCAACGC27650.040.7950174
CAACGCA28700.039.3025135
AACGCAG29900.038.5110976
TATCAAC35050.032.1846732
ACGCAGA36400.031.503737
GTACATG51850.029.0974351
CGCAGAG39300.029.0594438
ACATGGG52650.028.3892973
TACATGG53050.028.175242
GCAGAGT42550.026.6189569
AGAGTAC38200.023.92928910-11
GTACAGG5350.022.9125331
CATGGGG31500.022.6790084
TTAGACT3051.0913936E-1121.573434
GTCTTAG4600.021.5236031
GTATAAT4850.021.386241
CATGGGA25650.021.2550264
Produced by FastQC (version 0.11.2)