FastQCFastQC Report
Sun 14 Apr 2019
SRR6575971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245146
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18060.14504323187802876No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17030.13677110957269267No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15550.1248849532504622No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15160.12175279043582037No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14690.1179781326848418No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA14080.11307910879527382No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13700.11002725784767409No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13630.10946507477837941No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13080.10504792209106402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18400.064.384011
GGTATCA5550.051.6692471
TCAACGC26250.044.043214
ATCAACG26500.043.6277123
CAACGCA27550.042.1355365
AACGCAG28450.040.637416
TATCAAC33050.035.560172
ACGCAGA35100.032.80447
CGCAGAG37650.030.582598
GTACATG49300.028.416111
TACATGG50350.027.6367842
GCAGAGT41500.027.6321669
ACATGGG51550.026.5300143
AGAGTAC36450.024.30451610-11
GTCTTAG4050.023.2150841
CATATAG2555.6024874E-1022.1163883
CATGGGG31350.021.8871154
GGTACCT6850.021.2688648
AGTACAT34300.020.82680512-13
GTCTTAT2501.069202E-820.6846391
Produced by FastQC (version 0.11.2)