FastQCFastQC Report
Sun 14 Apr 2019
SRR6575969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011344
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13750.13595769589773607No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12360.12221360882152857No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10630.10510765871948614No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10610.10490990207090764No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10160.10046037747789081No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10130.10016374250502301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17750.067.657411
ATCAACG23950.049.8374633
GGTATCA5300.049.760611
TCAACGC24100.049.5272674
CAACGCA24700.048.1339235
AACGCAG25550.046.900456
TATCAAC31350.038.525192
ACGCAGA31900.037.4171567
CGCAGAG34450.034.669818
GCAGAGT36600.032.6331949
GTATTAT2950.028.735851
GTACATG41200.028.2340551
TACATGG41850.027.6241442
AGAGTAC32900.027.01300210-11
GTATTAC1606.780465E-826.4908621
ACATGGG43000.026.3376083
ACCTAAG5150.022.8615841
AGTACAT29350.022.42987612-13
CATGGGG24750.022.0247254
TAGGTAT3550.021.1796785
Produced by FastQC (version 0.11.2)