FastQCFastQC Report
Sun 14 Apr 2019
SRR6575968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114980
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13580.12179590665303414No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12540.11246838508314051No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11980.1074458734685824No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11340.1017058601948017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5800.068.2199551
GTATCAA20400.061.651161
TCAACGC28550.042.8008544
ATCAACG29150.042.0829963
CAACGCA29900.041.182755
AACGCAG31300.039.641026
GTATACC651.4133315E-436.1544153
TATCAAC35050.035.267322
ACGCAGA38000.032.650227
TAGGTAT3600.032.637945
CGCAGAG39150.031.57118
GTACATG48050.029.8996831
GCAGAGT42900.029.0304859
ACATGGG49500.028.7701513
TACATGG49350.028.6671162
CTTAGGT4000.028.2004453
AGGTATA3900.027.7171426
GGTATAG3950.027.3650677
TTAGGTA4200.026.8563614
CTATTAA2850.026.4444961
Produced by FastQC (version 0.11.2)