FastQCFastQC Report
Sun 14 Apr 2019
SRR6575964_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575964_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106984
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14320.12936049662867757No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13200.11924291588676982No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12440.1123774146690467No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12220.11039003273760055No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12180.1100286905682467No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11600.10478922911261589No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11210.1012661429614159No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11130.10054345862270818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17300.059.958391
GGTATCA6300.050.891091
ATCAACG23350.043.8840523
TCAACGC23750.043.144954
CAACGCA25350.040.2363785
AACGCAG26300.038.9616976
TATCAAC30550.033.5414922
TACGGTA600.004159076631.3361644
ACGCAGA34050.029.9489527
GTACATG43650.029.4884411
GTATAGG2800.028.626241
CGCAGAG35800.028.4849648
TACATGG44950.028.129352
ACATGGG45050.027.5452183
CATATAG1205.838823E-627.4191443
TTACGGT700.00883198926.859573
GTATAGA1257.828616E-626.4034941
TAGTACA1258.017936E-626.3223784
GCAGAGT38950.026.3019569
TATAGGG1101.0176778E-425.638682
Produced by FastQC (version 0.11.2)