FastQCFastQC Report
Sun 14 Apr 2019
SRR6575964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106984
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13420.12123029781821598No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13370.12077862010652367No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13120.11852023154806213No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12590.11373244780412364No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11980.10822197972147746No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11630.10506023573963129No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11290.10198882730012358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16550.062.7800941
GGTATCA4650.050.5527421
ATCAACG25000.041.547283
TCAACGC25050.041.462484
CAACGCA26250.039.746095
AACGCAG27650.037.90366
TATCAAC31550.032.932192
ACGCAGA34600.030.4258447
CGCAGAG35900.029.3240748
GTACATG44900.028.5853821
TAGGTAT3150.028.3474355
GCAGAGT38800.027.3745779
TACATGG46800.027.3244062
ACATGGG46900.027.05713
AGAGTAC33850.026.37944610-11
GTCTTAG5550.026.2601011
CTAAGAC7900.026.1767643
GGTACCT6750.025.7613548
TAAGACA8600.025.6844434
ACCTAAG5900.025.4991461
Produced by FastQC (version 0.11.2)