FastQCFastQC Report
Sun 14 Apr 2019
SRR6575962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818047
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12170.1487689582627893No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11400.13935629615413295No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10890.1331219355367112No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10590.12945466458528665No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10170.12432048525329228No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10010.12236460741253254No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9950.12163115322224763No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9820.12004200247663031No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9710.11869733646110799No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9620.1175971551756806No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9300.11368539949416111No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9290.11356315712911362No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9280.11344091476406612No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9230.1128297029388287No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8920.10904018962235666No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8780.10732879651169187No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8520.10415049502045726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19100.055.1409341
ATCAACG25500.040.1781463
GGTATCA8200.040.136881
CAACGCA25650.040.126415
TTAGGTA1305.456968E-1239.7670064
TAGGTAT1305.456968E-1239.7670065
AACGCAG30400.035.8664176
TATCAAC29650.035.52082
TCAACGC30350.033.9124374
ACGCAGA30900.033.3088157
CGCAGAG31400.032.9280938
GCATATA1052.0584175E-631.3449941
GTATTAC753.267585E-431.3449921
AGGTATA1658.731149E-1131.3315816
GTACTAG4250.030.9762271
GCAGAGT34050.030.365419
CTTAGGT1952.0008883E-1128.921463
GTACATG36350.028.1975571
ACATGGG36050.027.8985793
CAGTACG856.816626E-427.6455129
Produced by FastQC (version 0.11.2)