FastQCFastQC Report
Sun 14 Apr 2019
SRR6575961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570628
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA11100.19452252605900866No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8350.1463300083416867No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC7560.13248561234289238No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7170.12565103710298128No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7100.12442431847017672No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6990.12249661776148385No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6900.12091940809073512No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6850.12004318049587473No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6750.11829072530615392No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6490.11373434181287984No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6460.11320860525596362No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6400.11215713214213112No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6360.11145615006624279No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC6260.10970369487652201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.10795123968680122No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6010.10532255690222No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG5980.10479682034530377No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG5860.1026938741176388No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA5790.10146715548483426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA750.062.64857
GTATCAA14850.049.6976051
ACGTTAA1052.3646862E-1144.7489288
CAACGCA19850.035.9794925
ATCAACG20150.035.6773
AACGCAG21300.034.6331526
ACGCAGA22050.032.1766137
TATCAAC23400.031.5388642
TCAACGC22700.031.4622424
TCGAAAA300.00411000131.4123348-49
TCTTAGG1807.2759576E-1231.337982
GTATAAC902.5890266E-531.337981
ACCTAAG1950.031.3379781
TCTAACG1950.031.3379782
CGCAGAG22850.031.0500778
CCTAAGA2450.030.6984312
CTAACGC2000.030.5411433
CGTTAAC1551.5206751E-930.3137879
CGCCTAC2051.8189894E-1229.796247
GCAGAGT24600.029.2232329
Produced by FastQC (version 0.11.2)