FastQCFastQC Report
Sun 14 Apr 2019
SRR6575960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623296
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10620.1703845364000411No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8610.13813661566895985No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8520.13669267891980696No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8110.13011474484033267No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7970.12786862100831708No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7360.11808193859739194No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7330.1176006263476743No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7290.11695887668138412No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7160.11487319026594106No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7150.11471275284936852No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7020.11262706643392546No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6930.11118312968477256No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6630.10637000718759626No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6460.10364257110586303No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6400.10267994660642776No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG6350.10187775952356505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.10059426019098469No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC6260.10043382277441215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16250.053.5157781
TAGGTAT802.456345E-741.124615
CAACGCA22650.036.9356355
ATCAACG23050.035.6829643
TATTAAG801.1566015E-535.2553252
AACGCAG25000.033.8396846
TCAACGC25850.032.3633354
TATCAAC28000.031.0582642
ACGCAGA27150.030.4674857
CGCAGAG27500.030.4215328
GCAGAGT29450.028.5667959
GTACATG28800.028.0736851
GGTATCA6700.028.063941
TTAAGGT856.8129384E-427.6467974
AGAGTAC27050.027.62635810-11
TACATGG29350.027.5476022
AGGGCTA4950.027.5350935
ACATGGG29750.027.3308373
ACGGACG350.00883670726.85689256-57
CCGGTTA350.00883670726.85689292-93
Produced by FastQC (version 0.11.2)