FastQCFastQC Report
Sun 14 Apr 2019
SRR6575959_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575959_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458635
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8990.19601644008852356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.14194293937444807No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4890.10662073326283428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12700.038.1741751
GTACATG28150.034.946531
TACATGG28200.034.6646542
CATGGGT7150.034.1761134
ACATGGG29350.032.5022353
ATCAACG16150.030.2611983
AACGCAG16150.030.2611986
CATGGGG10950.030.0406464
GGGTACC4250.029.8538367
TCAACGC16450.029.7093224
CAACGCA16500.029.6192955
CCTACAC1652.9594958E-928.4800913
ATGGGTA4500.028.195295
TATCAAC18000.027.937272
GGTACCT4450.027.474068
ATGGGGA6900.025.8797345
TGGGTAC4750.025.7220176
ACGCAGA19250.025.3879667
GGACCGT950.001304659924.7327126
CGCAGAG20350.023.8002938
Produced by FastQC (version 0.11.2)