FastQCFastQC Report
Sun 14 Apr 2019
SRR6575959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458635
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9150.19950505303781874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.13213121545455536No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4840.10553054171617954No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4790.10444035016952478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12550.045.3077471
CTAGTAC1107.696872E-834.1728173
ATCAACG16500.033.3184973
CAACGCA16700.033.2008365
TCAACGC16700.033.2008364
AACGCAG17100.032.698996
GTACATG27250.032.4206851
TACATGG27250.032.0757832
ACATGGG28000.030.8775833
TATCAAC19100.029.2782042
ACTAATA650.006155001528.9186152
ACGCAGA20100.027.8185447
CATGGGT6250.027.8166754
TGTATAC1057.425313E-526.8500733
CGCAGAG20850.026.8178798
ATGGGTA4450.026.3975435
CATGGGG11550.025.2227954
GTATACA1551.5469268E-624.2516774
GCAGAGT23700.023.7912039
GGGTACC4050.023.2037667
Produced by FastQC (version 0.11.2)