FastQCFastQC Report
Sun 14 Apr 2019
SRR6575958_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575958_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562181
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8280.14728352612414863No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7900.14052413724405485No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7900.14052413724405485No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7870.13999050127983692No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7720.1373223214587473No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7710.13714444280400795No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7290.12967353930495695No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC6780.12060172791325213No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6730.11971233463955559No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6500.11562112558055146No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6330.11259718844998318No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6120.10886173670045768No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6080.10815022208150044No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6010.10690507149832527No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5990.10654931418884665No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG5690.10121295454666736No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA5630.10014568261823151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13000.059.756951
ATCAACG17800.042.505163
CAACGCA17700.041.683315
AACGCAG19850.039.0624246
GTATATA1101.7626007E-938.5210081
ACGCAGA20250.036.4375427
TCAACGC21000.035.5806274
CGCAGAG21050.035.0745858
TATCAAC23300.033.6908722
GCAGAGT22750.032.2469149
AGGGCTA4000.031.7204675
GGCTATT3700.030.4848427
GTACATG25500.030.4643251
TACATGG25600.030.113112
CTATTGA3600.030.0448679
GTACTAG3450.030.0228161
TAGGCAT3950.029.7425845
CTTTATA953.7457303E-529.6878912
GTATAAG3650.029.6676251
GGGCTAT3650.029.6122066
Produced by FastQC (version 0.11.2)