FastQCFastQC Report
Sun 14 Apr 2019
SRR6575958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562181
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8330.1481729193978452No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8190.14568261823149484No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8000.14230292379144796No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7910.14070201589879416No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7830.13927898666087968No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7710.13714444280400795No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7620.13554353491135418No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC7320.13020717526917486No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7010.12469293697225627No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6960.12380354369855973No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6920.12309202907960248No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6530.11615476154476939No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6400.11384233903315837No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6380.11348658172367974No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6340.1127750671047225No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6130.10903961535519699No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA5910.10512628495093217No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA5850.1040590130224963No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG5830.10370325571301768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13150.057.55741
CAACGCA16850.044.340965
ATCAACG17700.042.4770743
GTACTAG2900.042.1479451
AACGCAG19150.039.260796
CTTAGGT608.8290995E-539.158553
TCAACGC20050.037.498464
TATCAAC20750.036.479382
TATATAA1551.8189894E-1236.3989832
ACGCAGA20750.035.7805377
CGCAGAG21200.035.021048
TAGGCAT3350.033.6646655
GGTATCA5050.033.5129131
TCTACAC1001.4117486E-632.893183
GCAGAGT23150.031.8652929
AATAGGG753.267164E-431.3435692
TATAGTG902.585806E-531.3435672
GTACTAC753.2688698E-431.340781
CTATTAT1701.2187229E-1030.4189911
CTAGGCA3950.029.7406714
Produced by FastQC (version 0.11.2)