FastQCFastQC Report
Sun 14 Apr 2019
SRR6575954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320908
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC4800.14957557929375398No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT4740.14770588455258205No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG4620.1439664950702382No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA4580.14272003190945692No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA4290.13368317399379262No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4230.13181347925262069No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4220.13150186346242537No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4110.12807408977027682No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG3810.11872561606441723No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3800.1184140002742219No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT3770.11747915290363593No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3430.10688421603699504No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC3260.10158674760367457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7450.058.024781
TGCTAGA250.005238142356.3849452
CAGTACG250.005238142356.3849459
TTAAGGT708.6009095E-846.9874574
CAACGCA10650.041.9136925
GGTATCA2600.041.565831
ATCAACG10900.040.0902143
TATCAAC11250.038.42532
TAGGTAT500.001702430737.5899625
AACGCAG12600.036.9187166
TCAACGC12400.036.3773884
CTAGACA550.002717658634.1726954
TATTAAG1107.6848664E-834.1726952
ACGCAGA13550.033.290017
CGCAGAG13750.032.8057868
CTATTGA2450.032.603549
CAGTAGT2450.032.6035494
GTACTAG2200.032.0369031
TACGGCT600.004161987531.324974
GGGCTAT2750.030.7554286
Produced by FastQC (version 0.11.2)