FastQCFastQC Report
Sun 14 Apr 2019
SRR6575953_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575953_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413809
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6440.15562735464912555No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5590.13508647709450497No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC5490.13266990326454958No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5250.1268701260726567No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA5200.12566183915767903No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC5170.12493686700869241No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5150.12445355224270135No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5020.12131200626375938No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG5000.12082869149776829No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT4830.11672051598684417No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4570.11043742402896022No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA4220.10197941562411644No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4170.10077112870913876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10200.057.7693631
CAACGCA13600.043.887285
ATCAACG13400.043.8461573
GTACTAG2300.038.9415741
TCAACGC15600.038.2653274
AACGCAG16350.037.080526
ACGCAGA16000.036.4229857
CGCAGAG16550.035.496538
GCAGAGT16850.035.422379
TATCAAC17200.034.979042
ACGTTAA550.002715744834.1799288
GGTATCA4050.033.7544251
TAGGCAT2850.032.9806375
GTATAAG3050.030.9113451
GTGATCG2800.030.2126148
GTACATG18800.029.5877481
ACATGGG18400.029.3769253
AGGGTAA804.777203E-429.3733756
CTAGGCA3550.029.128674
CGTTAAC1154.0921223E-628.6937921
Produced by FastQC (version 0.11.2)