FastQCFastQC Report
Sun 14 Apr 2019
SRR6575953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413809
Sequences flagged as poor quality0
Sequence length100
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC6100.14741100362727733No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6090.14716934624428177No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA5620.13581144924349156No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG5500.13291156064754514No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT5400.13049498681758975No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5160.12469520962569687No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5160.12469520962569687No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4610.11140405356094238No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4510.108987479730987No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG4400.10632924851803609No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4380.10584593375204503No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA4330.10463764683706735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9400.058.006861
CAACGCA13350.040.486795
ATCAACG13350.040.486793
GTATCAG608.8081506E-539.17131
GGTATCA3600.037.8655931
GACTAAG651.410945E-436.158121
AACGCAG15450.035.592146
TCAACGC15300.035.3267064
TATCAAC15750.034.619972
AGGGCTA2750.034.181735
GTATAAG1950.031.337041
ACCTAAG1201.64624E-731.3370381
ACGCAGA17300.031.2426957
CGCAGAG17150.031.2419018
TGATCGC1859.094947E-1230.486419
GTGATCG1859.094947E-1230.486418
GGGCTAT2700.029.5925166
CTATTGA2700.029.5925169
GGCTATT2700.029.5925167
TATGCGG804.7757293E-429.3749245
Produced by FastQC (version 0.11.2)