FastQCFastQC Report
Sun 14 Apr 2019
SRR6575951_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575951_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730652
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17520.23978583511712828No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12860.17600718262592863No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC8860.12126155816996327No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG8230.11263912231814872No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.1052484630165934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17150.064.518311
GGTATCA4700.062.1116451
TCAACGC24550.044.9875564
ATCAACG25100.044.0047873
CAACGCA25100.044.0017785
TATCAAC25750.043.259042
AACGCAG25800.042.8079266
ACGCAGA31000.035.4756367
CGCAGAG32050.034.4624068
GGTAATC1755.456968E-1232.2291568
GCAGAGT35900.030.89759
AAGGGTA2203.6379788E-1227.7713345
GTACATG38550.027.7255821
CTAAGAC7700.026.8576343
GGACCGT700.00883611726.8557956
ACATGGG40200.026.4233673
AGAGTAC32950.026.0323410-11
ACCTAAG6950.025.7441181
TACATGG41400.025.6574752
ATGGGGG6650.025.4423315
Produced by FastQC (version 0.11.2)