FastQCFastQC Report
Sun 14 Apr 2019
SRR6575950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942370
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11760.12479174846397913No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10170.10791939471757378No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9820.10420535458471726No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9760.10356866199051327No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9570.10155246877553402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15150.060.4875341
GGTATCA4450.047.5221861
TCAACGC20300.044.4406054
ATCAACG20800.043.1464233
CAACGCA21300.042.3541955
AACGCAG21600.042.2016
TATCAAC26350.033.7073862
ACGCAGA26900.033.536967
CGCAGAG29300.030.9502748
TAAGACA7400.029.8428944
ACCTAAG4800.029.3713531
GCAGAGT31350.028.4767769
GTACATG36800.028.0943361
CTAAGAC7400.027.9380283
ACATGGG37750.027.2585013
TACATGG37600.027.2466162
TAGTCCG700.00884744526.8495335
TAGGTAT3350.026.6491625
ACGGTAC909.532156E-426.1037123
TTAGGTA3550.025.1478024
Produced by FastQC (version 0.11.2)