FastQCFastQC Report
Sun 14 Apr 2019
SRR6575949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072395
Sequences flagged as poor quality0
Sequence length100
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11920.11115307326125168No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11850.11050032870350943No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11400.10630411368945211No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10930.10192140023032557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17300.066.8510361
TCAACGC25000.045.3058624
ATCAACG25100.044.9381263
GGTATCA4850.043.620431
CAACGCA26250.042.9694025
AACGCAG27100.041.795086
TATCAAC31650.036.2439272
ACGCAGA33950.033.2237667
CGCAGAG36550.030.6032098
GTACATG44350.029.5753381
ACATGGG44950.028.9619273
TACATGG45050.028.8027152
GCAGAGT39500.028.3176529
AGAGTAC34400.026.23132110-11
ACCTAAG4500.024.0289331
CATGGGG26250.023.2750934
CCCTAAG1852.806828E-722.8712521
ACCAGAT6150.022.16155894
CATATAG1503.2923053E-521.93233
AGTACAT30850.021.86120812-13
Produced by FastQC (version 0.11.2)