FastQCFastQC Report
Sun 14 Apr 2019
SRR6575947_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575947_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888759
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16540.186102194183125No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12610.14188323268737646No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10940.12309298696271992No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10270.11555438538456432No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10180.11454173741137924No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10140.11409167164551921No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10060.11319154011379913No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9950.11195385925768403No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9510.10700313583322363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17000.060.632471
GGTATCA5800.053.5582051
TCAACGC22200.046.3597344
ATCAACG22500.045.5327383
CAACGCA22850.045.0409665
AACGCAG23650.043.318676
TATCAAC27300.038.21772
ACGCAGA28650.035.7586947
TAGGTAT3250.034.7038465
CGCAGAG30100.034.21348
GCAGAGT34500.030.1225579
ACCTAAG6350.028.906861
GTACATG38250.028.5474031
TACATGG38900.028.1501392
ACATGGG39000.027.835383
GTCTAGG1205.7775524E-627.4553411
AGGTATA3800.027.2075166
TATAGGG1258.024628E-626.3185672
TTAGGTA3750.026.3170834
GTATAGG2156.548362E-1126.2695621
Produced by FastQC (version 0.11.2)