FastQCFastQC Report
Sun 14 Apr 2019
SRR6575947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888759
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16010.18013882278547952No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11880.13366953246043078No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10350.1164545169162844No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9620.10824081668933873No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9520.10711565227468864No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9140.1028400274990183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14400.063.96331
GGTATCA4150.057.7509771
ATCAACG21250.044.8875123
TCAACGC22000.043.5708354
CAACGCA22900.041.4480675
AACGCAG23200.041.114646
TATCAAC24300.038.2931062
ACGCAGA28200.033.658187
CGCAGAG30150.031.7929828
ACCTAAG6100.030.0449891
TATAGGG1102.969371E-629.9066032
ATATGCG650.006161007628.915793
GCAGAGT33000.028.760839
GTACATG38600.027.7577841
TACATGG38400.027.6591572
ACATGGG38750.027.5259633
CTAAGAC7350.027.489673
TAAGACA7600.027.2036694
GTATAAG2008.1308826E-1025.8463951
AGAGTAC29600.025.71504410-11
Produced by FastQC (version 0.11.2)