FastQCFastQC Report
Sun 14 Apr 2019
SRR6575946_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575946_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984980
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17240.1750289345976568No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13910.14122114154602125No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12590.12781985421023775No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11000.11167739446486223No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10650.10812402282279843No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10630.1079209730146805No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10430.10589047493350118No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10200.10355540214014498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17550.063.903241
GGTATCA5000.053.7189981
TCAACGC24500.045.0815624
ATCAACG25250.043.5563623
CAACGCA25500.043.311465
AACGCAG26550.041.9526026
TATCAAC29250.038.564042
ACGCAGA32250.034.246277
CGCAGAG35000.031.5554898
GCAGAGT37950.029.22649
TACATGG44300.028.539462
GTACATG44450.028.410971
ACCTAAG8150.028.3309751
ACATGGG44800.028.3258483
ACCAGAT8250.027.34541194
CTAAGAC9000.026.6332913
CCTAAGA9200.026.0543062
AGAGTAC33550.025.70506310-11
AACGCGT551.01870915E-425.63632272-73
TAAGACA9550.024.6072884
Produced by FastQC (version 0.11.2)