FastQCFastQC Report
Sun 14 Apr 2019
SRR6575945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579505
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12050.20793608338150663No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11010.18998973261662971No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10720.1849854617302698No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9540.16462325605473635No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8490.14650434422481257No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8310.14339824505396848No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8070.13925677949284304No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC7740.13356226434629556No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7150.12338116150852883No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7080.12217323405320057No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6980.12044762340273166No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6930.11958481807749718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.10957627630477736No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6340.10940371523973046No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6260.10802322671935531No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6210.10716042139412084No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT5950.10267383370290162No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC5930.10232871157280783No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC5840.10077566198738579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16600.054.661441
CAACGCA21350.042.0344125
GGTATCA6850.041.866931
ATCAACG22100.040.6079033
AACGCAG24150.038.9118926
CGTTAAC1500.037.6116071
ACGCAGA24050.037.3153727
CGCAGAG24650.036.4070858
TCAACGC25400.035.517064
TATCAAC25750.035.2380562
ACTGCGC3600.033.934418
GCAGAGT26600.033.561519
ATACCGA753.2792316E-431.3240746
ATAAGAC600.004165315531.3240723
ATTATTA3650.030.9136471
ATTAACT3800.029.6754364
TGCGAGT3900.028.9145394
ACGCAAG650.006160574528.9145288
ATAAGGT4100.028.6500683
GTATATA1154.181613E-628.6175251
Produced by FastQC (version 0.11.2)