FastQCFastQC Report
Sun 14 Apr 2019
SRR6575942_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575942_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125675
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13390.1189508517111955No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12360.1098007861949497No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11960.10624736269349501No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11850.10527017123059497No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11320.10056188509116751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19250.065.404561
GGTATCA5550.059.4747351
ATCAACG28800.042.599393
TCAACGC29400.042.049784
CAACGCA29950.041.7465745
AACGCAG30800.040.7470866
TATCAAC34400.035.9378972
ACGCAGA37550.033.4179237
CGCAGAG39850.031.3712258
GCAGAGT42550.029.4897169
GTACATG49300.028.9816531
TACATGG49950.028.1378572
ACATGGG50600.027.497713
AGAGTAC37600.026.31013910-11
ACCTAAG5000.023.5774821
AGCCGAT1602.0387579E-623.49997994
TAAGACA7650.023.3494954
AGTACAT33150.023.10905612-13
CATGGGG30650.022.8513134
CATGGGA20600.022.8185544
Produced by FastQC (version 0.11.2)