FastQCFastQC Report
Sun 14 Apr 2019
SRR6575942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125675
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14650.13014413574077777No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14140.12561352077642304No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14060.12490283607613209No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12900.11459790792191353No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12650.11237701823350434No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12070.10722455415639505No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA11910.10580318475581318No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11470.10189441890421302No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11270.10011770715348568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19100.062.7629241
GGTATCA5850.053.841271
TCAACGC27000.043.8649254
ATCAACG27150.043.6225783
CAACGCA27900.042.618385
AACGCAG29050.040.9312486
TATCAAC32850.036.206082
ACGCAGA34650.034.587377
CGCAGAG36900.032.4783868
GTACATG44650.030.9543911
TACATGG45850.030.0417122
GCAGAGT41000.029.5744389
ACATGGG46750.028.852333
CATGGGG28600.025.3066864
AGAGTAC36700.024.9717610-11
GTCATAC1901.32968125E-824.7424551
AGTACAT30250.022.06193412-13
GTCTTAG5000.021.6249051
GTATAAT3553.6379788E-1219.8636591
ACCTAAG5500.019.6590061
Produced by FastQC (version 0.11.2)