FastQCFastQC Report
Sun 14 Apr 2019
SRR6575940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801617
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27580.34405457968082015No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15420.19236118994482404No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10950.1365988994744373No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10870.1356009166472268No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.1317337331917861No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10510.13110999392477954No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10050.12537159266831915No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9820.12250239204008896No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9150.11414428586220102No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8600.10728315392512884No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8540.10653466680472096No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8230.10266748334928028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24300.061.9017941
GGTATCA7200.054.8411181
CAACGCA34000.043.9568375
ATCAACG33800.043.9388473
TCAACGC34800.042.6762354
TATCAAC36250.041.625222
AACGCAG36250.041.358136
ACGCAGA42200.035.5268337
CGCAGAG42950.034.906468
CGTTATA550.002714629334.1866722
GCAGAGT45650.032.8418929
ACCAGAT10400.030.72938294
ACGTTAT650.006148804428.9271851
ACCTAAG10550.028.5158981
AAGGGTA3800.027.2092975
AGAGTAC43700.027.04797710-11
ATGGGAG9050.027.0043055
GTACATG50350.026.6075521
GTGCAAG4100.026.3695961
TAGTACT2700.026.1099344
Produced by FastQC (version 0.11.2)