FastQCFastQC Report
Sun 14 Apr 2019
SRR6575937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299633
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5130.17120944622254558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.1365003187232381No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3000.10012248317107929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG301.370224E-462.6601074
CGCTAAA250.005237900756.384685
GTATCAA9050.050.898191
CGCGCGT450.001014109541.7664267
ATCAACG11400.039.9870383
TCAACGC11650.039.5323374
CAACGCA11650.039.525745
AACGCAG12050.038.6036156
GTAGCGC500.00170089437.596063
GCGCGTG500.001702289337.5897878
GTACATG18750.035.847561
TACATGG18450.035.6661722
ACATGGG18100.035.5708583
GGTATCA4100.035.5387951
TATCAAC12950.035.2068252
AGCGCGC550.002717434734.1725355
ACGCAGA13700.033.6113057
GCAAACG702.1803656E-433.5735171
CGCAGAG14000.032.8910648
TGCCCGC753.2686142E-431.3352831
Produced by FastQC (version 0.11.2)