FastQCFastQC Report
Sun 14 Apr 2019
SRR6575936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981260
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10780.10985875303181622No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10740.10945111387399874No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10690.10894156492672687No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10650.10853392576890937No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10400.10598618103254999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.061.7230641
GGTATCA5150.052.0213661
ATCAACG19800.040.5800173
TCAACGC19850.040.4778024
CAACGCA21000.038.4849135
AACGCAG22300.036.2413946
TATCAAC25450.031.9501152
GTACATG37550.029.7907281
ACGCAGA27500.029.3884757
CCCTATA804.7793315E-429.3760992
TACATGG38900.028.2735522
ACATGGG39400.027.7869573
CGCAGAG30050.027.0509788
ACCTAAG3050.026.1977041
CTATACA1807.516064E-926.1041054
GCAGAGT32300.025.0211479
AGAGTAC27700.024.59628710-11
CATGGGG24850.023.824594
TAAGACA5850.023.2928924
TACCGTC5650.023.2857847
Produced by FastQC (version 0.11.2)