FastQCFastQC Report
Sun 14 Apr 2019
SRR6575935_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575935_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061838
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12310.11593105539639756No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11100.10453572013810015No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10650.10029778553790691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16950.060.8464051
GGTATCA5950.056.1955451
TCAACGC23900.043.263884
ATCAACG24100.042.709823
AACGCAG25600.040.5744826
CAACGCA25650.040.495395
TATCAAC29200.035.7331122
ACGCAGA31350.032.9811177
ACCTAAG3000.031.3956341
CGCAGAG34200.030.2326938
TACATGG42150.029.7724382
GTACATG42950.029.275791
ACATGGG42800.028.3319593
GCAGAGT37300.027.8460569
AGAGTAC31850.024.93776310-11
CTAAGAC6150.024.4554443
TTAAGAC1652.6633697E-622.7880293
TAAGACA6000.022.7168164
CATGGGG26600.022.6166884
CTATAGT1050.002308581322.4254531
Produced by FastQC (version 0.11.2)