FastQCFastQC Report
Sun 14 Apr 2019
SRR6575935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061838
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12440.11715534761423116No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11140.10491242543589513No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11040.10397066219140774No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10750.1012395487823943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15800.058.309761
GGTATCA6000.055.622351
TCAACGC20750.043.9404564
ATCAACG21200.042.7860643
CAACGCA21850.042.1585355
AACGCAG23500.039.1984676
TATCAAC27700.032.7506372
TTAGGTA2600.032.537194
GTACTAA600.0041587631.3365351
CTTAGGT2250.031.332113
ACGCAGA29650.031.0679287
TAGGTAT2250.029.2433035
CGCAGAG32100.028.9895238
GTACATG42750.028.9175741
GGTACCT6550.028.7011688
TACATGG44350.028.0862982
GCAGAGT33600.027.6953459
ACATGGG44750.027.6212673
GGGTACC7050.026.6656277
AGGTATA2500.026.3189726
Produced by FastQC (version 0.11.2)