FastQCFastQC Report
Sun 14 Apr 2019
SRR6575933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126646
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA38150.3386156787491368No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC34400.305331044533953No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29820.2646794112791418No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17680.15692595544652002No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16790.14902640225944974No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14180.12586029684568178No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG14120.12532774269823885No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13730.12186614073985973No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG13040.1157417680442659No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12520.11112629876642707No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT11960.10615579339029296No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11540.10242791435819236No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11470.10180660118617561No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11270.10003142069469914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38350.045.8404661
ATCAACG46700.037.337223
TCAACGC47200.036.94174
CAACGCA47750.036.7130435
AACGCAG49100.035.895066
GTACTAG1055.1073584E-835.813231
TATCAAC52850.032.907852
TAGGTAT8350.032.082885
GTCTTAG9550.031.0084441
AGGTATA8550.030.7827116
GTACATG78850.030.5218641
GGTAATC3750.030.0791058
ACGCAGA58350.030.0436657
TTAGGTA8850.029.739234
TACATGG81050.029.5193982
CGCAGAG59600.029.4135558
ACATGGG79900.029.2932473
AAGGGTA3700.029.2153455
GGTATAG8900.029.044087
CTTAGGT9200.028.6078453
Produced by FastQC (version 0.11.2)