FastQCFastQC Report
Sun 14 Apr 2019
SRR6575932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693596
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18190.2622564143968535No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13540.1952145052739635No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13280.19146592540902774No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10080.14532955784058732No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9230.13307458520522034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.13293040905656897No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8230.11865697034008268No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7700.11101563446155975No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7600.10957387297504599No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7070.10193253709652306No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC6950.10020242331270654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC652.7886417E-643.372573
AGGTATA2900.035.6452756
CTTAGGT3300.034.172333
GTATCAA34150.033.7241131
TAGGTAT3200.032.3035285
TAGGCAT2400.029.3668465
GGTAATC1952.0008883E-1128.9150478
GTACTAG1853.274181E-1027.950281
GTCTTAG5150.027.3828851
CAACGCA41800.027.3153825
GGTATAG3800.027.202977
CATGGGG21200.027.0396614
TCAACGC42650.026.8811634
AAGGGTA1755.5624696E-926.8496885
GTACATG60100.026.7495691
AACGCAG43250.026.6168866
ATCAACG43000.026.5530913
ACATGGG58650.026.3575553
TACATGG60950.026.29942
ATGGGAT6800.026.2574145
Produced by FastQC (version 0.11.2)