FastQCFastQC Report
Sun 14 Apr 2019
SRR6575931_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575931_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750708
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15920.2120664759134044No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14390.191685715351375No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12140.16171400864250815No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10460.1393351342998876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.13520569915333258No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9830.13094305642140486No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8160.10869738966415704No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8060.10736531381042962No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7920.10550040761521123No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7750.10323587866387463No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7580.10097134971253803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC1256.2645995E-933.8378643
GTATCAA33750.033.232371
TAGGTAT2750.032.470685
ATGGGAG9600.031.8209085
AGGTATA2750.030.7616986
ACCTAAG5300.029.342441
GTACATG63300.029.3326071
ACATGGG63300.028.8812753
TACATGG64400.028.606892
GGTACCT4450.028.5131598
GGGTACC4500.028.1963427
TTAGGTA3250.027.4751914
CTTAGGT3250.027.4751913
CATGGGG22400.027.2750664
TAGTACT1904.474714E-1027.208814
TCTAACG1057.4213865E-526.855452
TGGGTAC5200.026.2098876
GGTATAG3450.025.8807017
CAACGCA42700.025.7548165
AACGCAG43200.025.6743076
Produced by FastQC (version 0.11.2)