FastQCFastQC Report
Sun 14 Apr 2019
SRR6575928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044274
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23890.2287713760947797No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21280.2037779356758858No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15150.14507686679932663No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13330.12764849072178375No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13160.1260205654837715No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12280.11759365836935516No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11740.11242260173096331No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11270.10792186724940006No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10800.1034211327678368No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10780.10322961215160006No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10450.10006952198369393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4150.037.36431
TAGGCAT7750.027.8912455
TTAATAA5150.027.3745272
TAGGTAT2357.2759576E-1225.994855
CTAGGCA8150.025.9457764
CATGGGG26050.025.6148854
ACCTAAG3750.025.0605011
TAATAAT5700.024.731943
GTTAATA5200.024.3978421
CTAACTG1951.7598722E-824.0977884
CCTAACC1201.8403595E-423.4953443
GGTCTAT1401.9347764E-523.4942191
CAGTACG1000.001761582623.4930929
AGGTATA2653.6379788E-1123.0520346
GTTCTAA1050.00233869422.3754461
CCTAAGA5100.022.1143252
CTATTAT4250.022.1122061
TACATGG63550.022.0360262
GTATCAA42450.022.027561
GTACATG64050.021.9352741
Produced by FastQC (version 0.11.2)