FastQCFastQC Report
Sun 14 Apr 2019
SRR6575927_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575927_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124132
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21320.18965744236441984No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20610.1833414581205766No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17100.152117366999605No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15800.1405528888066526No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13970.12427366181195802No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13360.11884725281372649No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13090.11644539965057484No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12830.11413250401198435No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA12430.11057420302953745No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12400.11030733045585395No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11940.10621528432604001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG4650.030.4147741
GTACTAG5000.030.1714531
GCATATA1354.5131492E-727.9365311
TGGGTAC6350.026.6470186
TAGGCCG909.5147325E-426.1124325
CATGGGT9150.025.6843624
ATGGGTA7150.025.6376615
GGGTACC6750.025.0634757
CATGGGG26500.025.0088164
ACCGTCC950.001305535724.7336948
TAAGGTG4950.024.688124
ATAAGGT5050.024.1992453
GTGATCG5050.024.194948
GTCCTAC5350.023.7917441
TACACTA6150.023.6922575
GTATCAA38700.023.5105421
TCTAACG3200.023.501192
CGTTAAC2002.3012035E-823.497019
CACTATC5450.023.281447
TGATCGC5250.023.273239
Produced by FastQC (version 0.11.2)