FastQCFastQC Report
Sun 14 Apr 2019
SRR6575926_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575926_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320943
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4700.14644344945987292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.1420813041568129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7950.054.5159951
ATCAACG11600.036.8697743
TCAACGC11900.035.940284
CAACGCA12100.034.957815
AACGCAG12250.034.5297556
TATCAAC12750.033.1756442
GTACATG19150.032.9639551
TACATGG19050.032.0726932
TTCGCTA300.00416013231.33255482-83
ACATGGG19150.030.678093
TATTAAG1701.2187229E-1030.4110092
ACGCAGA14050.030.1060147
CGCAGAG14450.029.2726298
GGTATCA3350.028.1247351
CATGGGT6700.028.0590044
GCAGAGT15900.026.89879
GCGAAAT350.00883423826.85647464-65
ATGGGTA3450.025.8834155
ACTAGAC1301.0848546E-525.3070623
ATGGGAC2051.0750227E-925.2188855
Produced by FastQC (version 0.11.2)