FastQCFastQC Report
Sun 14 Apr 2019
SRR6575926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320943
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4730.14737819488195725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.125567468366657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8500.051.422931
GTATAAG1209.640644E-1139.166211
ATCAACG11950.035.790383
TCAACGC11950.035.790384
CAACGCA12100.035.7351235
AACGCAG12400.034.8705646
TACATGG20250.034.814412
ACATGGG20400.034.788813
GTACATG20350.034.6433331
TATCAAC13750.031.7887232
ACGCAGA13900.031.4456797
CGCAGAG13900.031.4456798
GTCCTAT1201.6461672E-731.3329681
CATGGGA10350.029.0624664
CATGGGT6500.028.1996734
CATGGGG6500.028.1996734
GGACAAT1005.3121712E-528.199676
GCAGAGT16000.027.6121799
CATTATA1205.8265214E-627.4163482
GTATAGT1205.8265214E-627.4163481
Produced by FastQC (version 0.11.2)