FastQCFastQC Report
Sun 14 Apr 2019
SRR6575925_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575925_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285667
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6710.234888874108665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.15927636023761932No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4550.15927636023761932No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4260.1491246801345623No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3890.13617253655479983No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3860.1351223627510353No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3410.11936975569456745No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3270.114468944610333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3110.10886801765692222No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2860.10011656929221786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10100.035.9507331
GTGTAAA1001.3702575E-633.0093081
GTACATG17200.032.3513151
GCCTTAT902.5291458E-531.437441
ATTAGAC600.004156099631.3328653
ACATGGG18050.031.2460733
TACATGG18400.030.9179742
TTAGGTA1551.5097612E-930.322134
ATCAACG12550.029.210723
TCAACGC12550.029.210724
TGTAAAC650.00613646928.9327752
CAACGCA12850.028.5337525
AGGTATA1503.67163E-828.2045176
TAGGTAT1503.67163E-828.2045175
TGGGTAC2500.028.2045176
GTCTTAG2053.45608E-1127.6036051
AACGCAG13550.027.4066056
CATGGGG9100.027.3732194
CCCCTAT700.00868345626.9463751
GTTCCTA700.00868345626.9463751
Produced by FastQC (version 0.11.2)