FastQCFastQC Report
Sun 14 Apr 2019
SRR6575924_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575924_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289801
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30520.2366256500033726No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30330.23515255454135947No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20610.15979209195837188No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20300.15738862041508728No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19870.15405477279053126No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT19250.14924782970396208No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18750.14537126269866435No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16580.12854696189567227No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16350.12676374107323535No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA16140.1251355829310103No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16030.1242827381898448No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15120.11722738624020294No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14590.11311822521458738No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT14510.11249797449373973No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14190.11001697161034918No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG14090.10924165820928966No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13960.10823375078791225No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT13940.10807868810770033No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA13760.10668312398579316No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG13760.10668312398579316No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13620.1055976852243098No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG13610.10552015388420384No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA13410.10396952708208475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA3650.033.474986
ACCTAAG4600.032.7484171
TTAGGTA3900.031.3267174
TAGGTAT3900.031.3267175
TCTTAGG5900.031.062442
GTACTAG3400.030.4608421
CTTAGGT4100.029.7985863
CGTTAAC1303.2854405E-728.9697511
TAGTACT5250.028.641574
CATGGGG31150.027.3040264
GTATAGT4950.025.6498458
GTCTTAG8500.024.9225061
GTCCTAC4000.024.7148191
TGGGTAC8400.024.6157596
TAAGACA8050.024.5165624
CCTAAGA6350.024.4209862
ATGGGGG14450.024.3893135
CCTAGTA6350.023.6809562
GGGTACC8750.023.6311267
TATTAAG2202.4574547E-923.4959492
Produced by FastQC (version 0.11.2)