FastQCFastQC Report
Sun 14 Apr 2019
SRR6575923_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575923_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411890
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28340.20072385242476398No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28130.19923648442867364No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23480.16630190737238737No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT20850.14767439389754158No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20810.1473910857078101No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20800.14732025866037723No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20370.14427469562076364No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG18650.1320924434623094No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17990.12741785833173971No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16530.11707710940654016No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA16200.11473981684125534No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15650.1108443292324473No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15470.10956944237865557No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15270.10815290142999809No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15190.1075862850505351No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA15080.1068071875287735No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT15050.10659470638647486No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG15000.10624057114931051No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG14910.10560312772241465No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT14650.10376162448915993No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA14620.1035491433468613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC1807.2759576E-1231.416191
GTACTAG4500.029.321781
TAGTACT6200.028.8069294
GTTTAGT1005.2294075E-528.274571
ACGTATA856.8190275E-427.645568
GGGTACC9100.027.3721437
CTAGTAC6750.027.1560063
TCTAACG3400.026.2651422
CATGGGG38300.025.8933774
CCTAGTA7200.025.4587542
CTTAGGT3550.025.1553463
TACATGG85700.024.8440532
TGGGTAC9650.024.8397926
GTATCAA49200.024.8072971
ACATGGG84650.024.5969813
GGTACCT10350.024.5204098
CATGGGT12550.024.343034
GTACATG87350.024.2230151
ATGGGTA9550.024.1155855
GTACTTG5500.023.9905451
Produced by FastQC (version 0.11.2)