FastQCFastQC Report
Sun 14 Apr 2019
SRR6575923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411890
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29370.20801903831034996No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24340.1723930334516145No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA23800.16856837289023932No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT22930.1624064197635793No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22290.1578734887278754No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA21400.15156988150634965No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT19980.14151244077088157No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19440.1376877802095064No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG17940.12706372309457536No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17650.12500973871902202No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA16840.11927274787695925No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG16520.11700628235910729No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT16330.1156605684578827No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15980.11318162179773213No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15760.1116234267542089No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15460.1094986153312227No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG15360.10879034485689394No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15240.10794042028769947No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT14500.10269921877766681No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14270.10107019668671072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC2300.034.747391
ACCTAAG4550.030.996381
GTACTAG5600.029.3819851
GTACCGA1005.326035E-528.1987156
TGGGTAC9000.027.6765166
GGGTACC8950.027.3060197
TCTAACG3800.027.2169972
CTAACGC3850.026.8559193
TTAACGT700.00883865826.8559194
TAATAAT4600.026.5640053
ATGGGTA10200.026.2635085
GGTACCT9300.025.2676648
TAAGACA8400.024.6179264
CCTAAGA6350.024.4310042
ACGCCTA4450.024.2910256
CTAAGAC7750.024.2569583
TAAGGGT1951.7580533E-824.1014654
TAGTACT7250.023.9851134
GTTAATA5150.023.7337991
TCTTAGG5400.023.5055892
Produced by FastQC (version 0.11.2)