FastQCFastQC Report
Sun 14 Apr 2019
SRR6575919_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575919_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294621
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21900.16916147660203257No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21250.16414070218233753No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19120.14768801062241382No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18360.14181756668553963No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18270.14112238253512033No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17950.13865061666696277No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT17500.1351746959148662No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16930.13077186296221055No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16140.1246696909751966No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15440.11926270313860196No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14530.1122336189510289No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14500.11200189090088913No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14300.11045703723329067No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA14180.10953012503273159No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13660.10551350549697555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT8400.033.012224
GTACTAG4350.031.4112761
CTAACGC3050.030.8199623
GGGTACC8500.030.410887
CATGGGT10950.030.0459444
CTGTGCG9500.029.6833449
CTAGTAC9150.029.2789653
TGGGTAC8800.028.841186
ACCTAAG3800.028.518131
CCTAGTA9800.027.8165912
GTACTGT10250.027.5124556
GGTACCT8900.027.4598718
ATGGGTA9300.027.2905795
TCTAACG3450.027.2466352
TACTGTG12850.026.3338627
ACGCCTA3850.025.6366066
GTACATG73300.025.5832611
TACATGG73050.025.543022
CATGGGG33600.025.3186914
CGATAAT5450.025.00844666-67
Produced by FastQC (version 0.11.2)