FastQCFastQC Report
Sun 14 Apr 2019
SRR6575919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294621
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24180.18677280841265514No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21960.1696249327023121No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20840.16097375216376067No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20310.15687988994462473No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19880.15355845455928802No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19070.1473017972055142No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC18560.14336242035313812No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18340.14166308131877978No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16540.12775939831039357No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16280.12575108854251554No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15990.12351105072449775No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15960.12327932267435797No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA15530.11995788728902125No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG15010.11594126775326524No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG14060.1086032128321725No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13780.10644041769753464No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA13020.10056997376066046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAC1452.6600901E-829.1730963
CTAACGC2800.028.5363833
TCTAACG2850.028.0400772
GTACTAG4800.026.4422041
ACGCCTA3200.026.4370986
TAGTACT7500.026.3206184
CGTTAAC2156.548362E-1126.232169
CCTACAC8400.025.7386973
GACGTTA2208.731149E-1125.6359737
ACCTAAG4050.025.5354061
GTCCTAC5550.025.4099271
TCCTACA7800.025.3121932
GTATCAA45800.025.0437561
GTACATG77100.024.876021
ACATGGG76700.024.7567753
TACATGG77800.024.65222
ACGTTAA2301.546141E-1024.5213688
GTACTGT9950.024.0900866
CACTATC5350.023.7192657
CATGGGT11650.023.3996894
Produced by FastQC (version 0.11.2)