FastQCFastQC Report
Sun 14 Apr 2019
SRR6575910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862548
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG26010.13964740774465947No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA24930.1338488994646044No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC24660.13239927239459065No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24610.13213082293718068No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT24550.13180868358828873No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA23920.12842622042492327No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23440.12584910563378768No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23040.12370150997450804No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT19710.10582277611100492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30400.050.8811
ATCAACG36700.041.2366333
TCAACGC36900.041.1393934
CAACGCA38600.039.4493145
GGTATCA13300.038.8843081
AACGCAG40550.037.6681446
TATCAAC49650.030.5855732
ACGCAGA50550.030.2164867
GTACATG71700.027.998971
TACATGG73450.027.3318732
GTCTTAG10150.027.328721
TTAGGTA6550.027.2661634
CGCAGAG56400.027.1656598
CTTAGGT6600.027.0603283
TTAACGC700.00883784526.8568673
TAACGCT1406.373957E-726.8561464
ACATGGG74050.026.657393
TAGGTAT6600.026.3475065
CCCTATA1659.296127E-825.6443612
GCAGAGT60400.025.6000449
Produced by FastQC (version 0.11.2)