FastQCFastQC Report
Sun 14 Apr 2019
SRR6575908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480528
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23940.16169906952114382No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21210.14325970194417126No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20700.13981498492429728No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20270.13691061567224666No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20090.13569483319464407No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19310.1304264424583662No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17990.12151070428928058No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT17080.11536424843028974No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16810.11354057471388584No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG15300.10334151059621974No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG15180.10253098894448466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22150.054.118171
GGTATCA8650.044.0195471
TCAACGC28500.041.7205124
ATCAACG28350.041.6097033
CAACGCA29400.039.8039365
AACGCAG30550.038.151756
CAACGCG500.001702645637.597935
TATCAAC38650.031.2579352
ACGCAGA39400.029.5821297
GTACATG58100.028.7229391
TACATGG57800.028.4653722
ACATGGG59900.027.2255483
CTTAGGT4500.027.1540623
CGCAGAG42850.027.0906938
AGGTATA4100.026.3644056
TAGGTAT4350.025.9296075
GGTACCT10550.025.3919688
GGGTACC10550.025.3919687
GTCTTAG7450.025.2394771
GCAGAGT47450.024.5634549
Produced by FastQC (version 0.11.2)