FastQCFastQC Report
Sun 14 Apr 2019
SRR6575904_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575904_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116014
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA53420.47866783033187754No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC37810.33879503303722No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28250.25313302521294534No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG17510.1568976733266787No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG17170.15385111656305386No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT17000.15232783818124146No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15040.13476533448505126No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC12880.11541073857496412No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12740.11415627402523625No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT12020.10770474205520719No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11920.10680869594825872No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA11890.10653988211617417No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT11850.10618146367339477No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA11320.10143241930656782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37950.043.2276151
GGTAATC3750.040.1039738
ATCAACG47100.034.4306073
TCAACGC47750.034.0588344
CAACGCA48100.033.9072045
TATCAAC48450.033.471242
AACGCAG48800.033.4208346
AAGGGTA5200.030.7314595
ACCTAAG20600.029.2911591
TCTAACG650.006150724428.926322
AGGGTAA5900.028.678616
GGTATCA19200.028.235171
CTAAGAC23350.028.1830273
ACGCAGA57150.028.1240967
GTACATG74100.027.6735481
TACATGG74650.027.4538462
TAAGACA24350.027.4104674
CGCAGAG58650.027.4048088
ACATGGG75250.027.1100143
ACCAGAT22200.027.09849494
Produced by FastQC (version 0.11.2)