FastQCFastQC Report
Sun 14 Apr 2019
SRR6575903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584049
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11760.20135296867214908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.1143739651981255No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6650.11386030966579859No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA5930.10153257688995272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12800.059.126181
ATCAACG18650.040.3209463
TCAACGC19050.039.967744
CAACGCA19850.038.356955
AACGCAG20400.038.013986
TATCAAC22300.033.72712
GGTATCA4500.033.4273071
TACATGG33450.032.4623372
GTACATG34150.032.0722271
ACGCAGA24050.031.658447
ACATGGG34350.031.6063823
CGCAGAG24650.031.0785148
GTATACG856.805989E-427.6512661
GTATTAA1555.066977E-827.2944741
GCAGAGT28050.026.6412019
CATGGGG16700.025.6102894
GTACATA1501.1593547E-625.070481
CATGGGA15250.024.9634574
ATGGGTA5700.024.7363685
AGAGTAC26500.024.29764710-11
Produced by FastQC (version 0.11.2)