FastQCFastQC Report
Sun 14 Apr 2019
SRR6575901_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575901_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483720
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37930.2556412261073518No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC35760.24101582508829159No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31090.20954088372469198No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16520.11134176259671635No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG16050.10817404901194295No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT15270.10291699242444667No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15260.1028495942630685No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14980.1009624457444801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26300.051.0581441
TCAACGC31950.040.898684
ATCAACG32600.039.9417273
CAACGCA32750.039.6125835
AACGCAG34100.038.0443426
GGTATCA11350.035.2857171
TATCAAC40150.032.6650472
ACGCAGA41450.031.5207927
GTCTTAG12000.030.6259271
CTTAGGT10450.030.5884363
GTACATG63850.030.4027021
TTAGGTA10350.029.9736044
TAGGTAT10100.029.7847545
TACATGG65500.029.280842
ACATGGG66750.028.802933
TCTTAGG13300.028.6284642
CGCAGAG45500.028.6118018
AGGTATA11100.027.101446
GCAGAGT50550.026.1253439
GGTATAG12150.025.5296297
Produced by FastQC (version 0.11.2)