FastQCFastQC Report
Sun 14 Apr 2019
SRR6575901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483720
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC35680.2404766397972663No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA34790.2344782034346103No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30670.20671016094680936No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18170.12246245922411236No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16660.11228533685601057No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16520.11134176259671635No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16490.1111395681125819No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG16210.10925241959399347No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15800.10648909497748903No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT15400.10379316852236271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26050.052.3361931
GGTATCA10450.040.4890941
ATCAACG33550.039.7837683
TCAACGC33850.039.431184
CAACGCA34550.038.6322825
AACGCAG35950.037.3892946
GTCTTAG11000.036.3277171
GTATACG651.4115064E-436.1633381
TATCAAC41150.033.017172
GTACATG67400.031.0393031
TTAGGTA10050.030.864414
TAGGTAT9950.030.7022615
TCTTAGG12950.030.494492
TACATGG68400.030.3105852
ACGCAGA44850.029.8649987
CTTAGGT10450.029.6829993
ACATGGG68800.029.4421083
AGGTATA10400.029.37386
CGTATAA650.006146848728.930672
GGTAATC3800.027.2094148
Produced by FastQC (version 0.11.2)